Early postnatal expression and localization of matrix metalloproteinases-2 and -9 during establishment of rat hippocampal synaptic circuitry
| Autor: | Paven K, Aujla, George W, Huntley |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Adhesion Molecules
Neuron-Glia Intracellular Signaling Peptides and Proteins Synaptophysin Gene Expression Regulation Developmental Membrane Proteins Hippocampus Article Rats Rats Sprague-Dawley Animals Newborn Matrix Metalloproteinase 9 Synapses Animals Matrix Metalloproteinase 2 RNA Messenger Nerve Net Disks Large Homolog 4 Protein |
| Zdroj: | The Journal of comparative neurology. 522(6) |
| ISSN: | 1096-9861 |
| Popis: | Matrix metalloproteinases (MMPs) are extracellular proteolytic enzymes that contribute to pericellular remodeling in a variety of tissues, including brain, where they function in adult hippocampal synaptic structural and functional plasticity. Synaptic plasticity and remodeling are also important for development of connectivity, but it is unclear whether MMPs--particularly MMP-2 and -9, the major MMPs operative in brain--contribute at these stages. Here, we use a combination of biochemical and anatomical methods to characterize expression and localization of MMP-2 and MMP-9 in early postnatal and adult rat hippocampus. Gene and protein expression of these MMPs were evident throughout hippocampus at all ages examined, but expression levels were highest during the first postnatal week. MMP-2 and MMP-9 immunolocalized to punctate structures within the neuropil that codistributed with foci of proteolytic activity, as well as with markers of growing axons and synapses. Taken together, discrete foci of MMP proteolysis are likely important for actively shaping and remodeling cellular and connectional architecture as hippocampal circuitry is becoming established during early postnatal life. |
| Databáze: | OpenAIRE |
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