| Autor: |
M, Gupta, M, Kaur, K G, Gupta |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Indian journal of experimental biology. 30(5) |
| ISSN: |
0019-5189 |
| Popis: |
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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