In vitro and in vivo induction of nitric oxide by murine macrophages stimulated with Bordetella pertussis

Autor:	D, Torre, G, Ferrario, G, Bonetta, L, Perversi, F, Speranza
Rok vydání:	1996
Předmět:	Mice Macrophages Alveolar Macrophages Peritoneal Animals Female Mice Inbred Strains Macrophage Activation Nitric Oxide Bronchoalveolar Lavage Fluid Administration Intranasal Bordetella pertussis Cells Cultured Cell Line
Zdroj:	FEMS immunology and medical microbiology. 13(2)
ISSN:	0928-8244
Popis:	Nitric oxide (NO) exhibits potent antimicrobial activity in vitro. The function of NO in host defenses in vivo, however, is presently unclear. Experiments were undertaken to determine the production of NO in vitro from murine peritoneal and alveolar macrophages, and murine macrophage cell line (J774A.1) stimulated with Bordetella pertussis or pertussis toxin (PT). In addition, we determined circulating levels of NO in the sera and bronchoalveolar lavage (BAL) fluids of mice infected intranasally with B. pertussis. The results of this study showed that in vitro murine peritoneal macrophages induce production of NO in response to B. pertussis and PT. In addition, murine macrophage cell line, J774A.1 also induces NO production after stimulation with B. pertussis. NO production was also detected in alveolar macrophages from mice infected intranasally with B. pertussis. Finally, a significant increment of circulating levels of NO was noted, in the sera but not in the BAL fluids, of mice infected intranasally with B. pertussis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::93b0e339e51dcf614afbbc9d2f432a37 https://pubmed.ncbi.nlm.nih.gov/8731016 Zobrazit plný text záznamu Plný text