| Autor: |
S N, Tamkovich, D S, Serdukov, O S, Tutanov, T G, Duzhak, P P, Laktionov |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Bioorganicheskaia khimiia. 41(6) |
| ISSN: |
0132-3423 |
| Popis: |
Circulating nucleoprotein complexes were isolated-from blood plasma by affinity chromatography using immobilized polyclonal anti-histone antibodies. It was found, that the main part of DNA from histone-contained nucleoprotein complexes have size 170-180 b.p., in blood of breast cancer patients DNA with size 170-180 b.p. and DNA more then 6 k.b.p. are presented in equal quantity. Proteins from circulating nucleoprotein complexes were identified using MALDI-TOF mass-spectrometry. It was shown that nucleoprotein complexes from blood of breast cancer patients contain tumor-specific proteins, such as LDOC1L, ADP/ATP translocase 3 and Lamellipodin. These data indicate, that a part of circulating extracellular DNA have tumor origin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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