Association of the duration of post-thaw culture with clinical outcome after vitrified-warmed day 3 embryo transfer in 10,464 cycles
| Autor: | Zhu, Haiyan, Xu, Wen, Jin, Xiaoying, Xue, Yamei, Tong, Xiaomei, Zhang, Songying |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Cryopreservation Time Factors Pregnancy Rate Observational Study post-thaw culture duration Embryo Transfer Vitrification live birth rate vitrified-warmed embryo transfer Embryo Culture Techniques Pregnancy Case-Control Studies Humans Female pregnancy rate Embryo Implantation implantation rate Research Article Retrospective Studies |
| Zdroj: | Medicine |
| ISSN: | 1536-5964 0025-7974 |
| Popis: | This study aimed to investigate the effect of the duration of embryo culture on clinical outcome in vitrified-warmed cycles. This retrospective cohort study enrolled 10,464 infertile patients, with a total of 18,843 vitrified-warmed day 3 embryos from 2012 to 2017 at a single center. The patients were divided into 2 groups: 9470 cycles in the short-term culture group (0.5–8 hours of post-thaw culture) and 994 cycles in the 48 to 72 hours culture group. The independent effect of the following variables on clinical outcomes was determined: duration of post-thaw culture, maternal age, transferred embryos, embryo quality, and endometrial thickness. We found that the pregnancy rate was positively associated with the post-thaw culture time. Ordinary least square regression analyses showed that the duration of post-thaw culture was positively associated with implantation and live birth rates overall. However, the implantation and live birth rates were not significantly associated with the post-thaw culture time in the short-term culture group. Additionally, maternal age and the number of transferred embryos were independent predictors of the implantation and live birth rates. Moreover, the duration of post-thaw culture did not affect live birth weight. These results indicated that the pregnancy rate is positively associated with the duration of post-thaw culture. Therefore, under the condition of not affecting work shifts, properly prolonging the duration of post-thaw culture to improve the outcome of frozen-thawed embryo transfer should be considered. |
| Databáze: | OpenAIRE |
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