Accelerated fibrillation of alpha-synuclein induced by the combined action of macromolecular crowding and factors inducing partial folding
| Autor: | L A, Munishkina, A L, Fink, V N, Uversky |
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| Rok vydání: | 2009 |
| Předmět: |
Anions
Models Molecular Time Factors Chemical Phenomena Dose-Response Relationship Drug Macromolecular Substances Static Electricity Hydrogen-Ion Concentration Oxidants Polyethylene Glycols Methylamines Microscopy Electron Metals Escherichia coli alpha-Synuclein Animals Drug Interactions Glycosaminoglycans Protein Binding |
| Zdroj: | Current Alzheimer research. 6(3) |
| ISSN: | 1875-5828 |
| Popis: | To better model the characteristics of crowded intracellular environments, we examined the effect of several factors known to induce partial folding and accelerated fibrillation of alpha-synuclein in dilute solutions, on the fibrillation of this protein in a crowded milieu. We found that low pH, certain metals and pesticides, polyanions, polycations and low concentrations of organic solvents cause a significant acceleration of alpha-synuclein fibrillation in the presence of high concentrations of polyethylene glycol. This suggests that the fibril-promoting effects of factors inducing partial folding of alpha-synuclein and the fibril-stimulating effects of macromolecular crowding are relatively independent and thus might act additively or even synergistically. |
| Databáze: | OpenAIRE |
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