| Autor: |
J Y, Yeh, B R, Ou, N E, Forsberg, P D, Whanger |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 10(1) |
| ISSN: |
0966-0844 |
| Popis: |
When rat L8 muscle cells were cultured to examine the effects of serum and selenium concentration on selenoprotein W levels and glutathione peroxidase (GPX) activities, no significant differences (P0.05) were found in selenoprotein W levels and GPX activities during differentiation. With three different forms of selenium, selenoprotein W levels and GPX activities were shown to increase in L8 myotubes cultured in media with these selenocompounds. Selenite was utilized more efficiently than selenocysteine for both selenoprotein W and GPX activity, but selenium as selenomethionine was less available. Both the protein content and mRNA levels for selenoprotein W were affected by the selenium content of the media. Northern blot data indicated that the expression of selenoprotein W mRNA increased significantly when L8 myotubes were cultured with selenium (P0.05). L8 myotubes cultured in 10% calf serum (CS) versus 2% CS with or without addition of 10(-8) M selenium indicated that the increase of selenoprotein W level in L8 myotubes cultured with higher serum concentration (10% CS) is due to the higher selenium concentration in media rather than serum itself. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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