Reed-Sternberg cell genome expression supports a B-cell lineage
| Autor: | J, Cossman, C M, Annunziata, S, Barash, L, Staudt, P, Dillon, W W, He, P, Ricciardi-Castagnoli, C A, Rosen, K C, Carter |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Expressed Sequence Tags
B-Lymphocytes DNA Complementary Cell Differentiation Dendritic Cells Germinal Center Hodgkin Disease Neoplasm Proteins Gene Expression Regulation Neoplastic Sequence Homology Nucleic Acid Tumor Cells Cultured Humans Cell Lineage RNA Messenger RNA Neoplasm Reed-Sternberg Cells Gene Library |
| Zdroj: | Blood. 94(2) |
| ISSN: | 0006-4971 |
| Popis: | The malignant Reed-Sternberg cell of Hodgkin's disease, first described a century ago, has resisted in-depth analysis due to its extreme rarity in lymphomatous tissue. To directly study its genome-wide gene expression, approximately 11,000,000 bases (27,518 cDNA sequences) of expressed gene sequence was determined from living single Reed-Sternberg cells, Hodgkin's tissue, and cell lines. This approach increased the number of genes known to be expressed in Hodgkin's disease by 20-fold to 2,666 named genes. The data here indicate that Reed-Sternberg cells from both nodular sclerosing and lymphocyte predominant Hodgkin's disease were derived from an unusual B-cell lineage based on a comparison of their gene expression to approximately 40,000,000 bases (10(5) sequences) of expressed gene sequence from germinal center B cells (GCB) and dendritic cells. The data set of expressed genes, reported here and on the World Wide Web, forms a basis to understand the genes responsible for Hodgkin's disease and develop novel diagnostic markers and therapies. This study of the rare Reed-Sternberg cell, concealed in its heterogenous cellular context, also provides a formidable test case to advance the limit of analysis of differential gene expression to the single disease cell. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|