[The induction of the antitumor activity of T-lymphocytes by antigen-presenting cells obtained from the blast cells of patients with acute leukemias]
| Autor: | V G, Savchenko, E Iu, Sadovnikova, E N, Parovichnikova, L P, Mendeleeva, I A, Demidova, A N, Sokolov, V G, Isaev |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Time Factors Antigen-Presenting Cells Pilot Projects Cell Separation Precursor Cell Lymphoblastic Leukemia-Lymphoma Cytotoxicity Tests Immunologic Flow Cytometry Immunotherapy Adoptive Lymphocytes Tumor-Infiltrating Phenotype Leukemia Myeloid Acute Disease Tumor Cells Cultured Humans Female T-Lymphocytes Cytotoxic |
| Zdroj: | Terapevticheskii arkhiv. 72(7) |
| ISSN: | 0040-3660 |
| Popis: | To produce antigen-presenting cells (APC) from tumor cells of patients with acute leukemia and to induce autologous cytotoxic tumor-specific T-lymphocytes in vivo and in vitro.Culture examinations, flow cytofluorometry were made in 3 patients with acute myeloid leukemia (AML) and 1 patient with acute lymphoblastic leukemia (ALL).Culturing of blast cells in the presence of growth factors produced APC in 4 patients with acute leukemia. The use of fibronectin layer with added collagen proved most effective for the above production. In culture, APC induced autologous tumor-specific T-lymphocytes which selectively lysed tumor cells. Pilot clinical trial was made of dendritic cells suspension in a patient with resistant AML.A new perspective immunotherapy of acute leukemia is proposed. |
| Databáze: | OpenAIRE |
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