| Autor: |
M F, Gulyĭ, N N, Orlovskaia, L D, Veselovskaia |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1. 59(5) |
| ISSN: |
0201-8470 |
| Popis: |
Amino acid compositions of aspartyl- and valyl-tRNA synthetases from the muscles of long-fasting and normal rabbits were studied. Certain differences in amino acid content of fasted and normal rabbits were found. The possibility of incorrect aminoacylation was shown for the tRNA and amino-acyl-tRNA synthetases (ARS) from the muscles of experimental animals. The Km values of incorrect reactions increased with specific and nonspecific amino acids depending on the decreased affinity between specific and nonspecific substrates. At the same time Vmax of these reactions decreased. A presumable decrease in the specificity of ARS isolated from muscles of long-fasting rabbits can be one of the reasons of synthesis of the proteins with the different amino acid composition by the extremal states of the organism. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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