A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays
| Autor: | Larry H, Stanker, Robert M, Hnasko |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Hybridomas
Immunoconjugates Luminescence Light Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity High-Throughput Screening Assays Immunoglobulin Fc Fragments Culture Media Conditioned Animals Humans Rabbits Botulinum Toxins Type A Horseradish Peroxidase Protein Binding |
| Zdroj: | Methods in molecular biology (Clifton, N.J.). 1318 |
| ISSN: | 1940-6029 |
| Popis: | The sandwich immunoassay (sELISA) is an invaluable technique for concentrating, detecting, and quantifying target antigens. The two critical components required are a capture antibody and a detection antibody, each binding a different epitope on the target antigen. The specific antibodies incorporated into the test define most of the performance parameters of any subsequent immunoassay regardless of the assay format: traditional ELISA, lateral-flow immunoassay, various bead-based assays, antibody-based biosensors, or the reporting label. Here we describe an approach for identifying monoclonal antibodies (mAbs) suitable for use as capture antibodies and detector antibodies in a sELISA targeting bacterial protein toxins. The approach was designed for early identification of monoclonal antibodies (mAbs), in the initial hybridoma screen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|