| Autor: |
F S, Steven, M M, Griffin, S A, Cederholm-Williams, W F, Mangel, H, Maier |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 11(2) |
| ISSN: |
0250-7005 |
| Popis: |
Tumour cells possess a cell surface protease referred to as guanidinobenzoatase (GB). The active centre of GB binds the fluorescent probe 9-amino acridine (9-AA) and this binding enables cells possessing active GB to be located by fluorescent microscopy. GB binding of 9-AA was inhibited by prior treatment of sections of tumour tissue with a specific polyclonal antibody recognising the tumour associated protease tissue plasminogen activator (t-PA). GB binding of 9-AA was also inhibited by prior treatment of sections of tumour tissues with PAI-I, a protein inhibitor of plasminogen activatory. We conclude from these studies and kinetic analyses that GB and t-PA are very similar both in structure and function. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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