[123I] beta-CIT, a tracer for dopamine and serotonin re-uptake sites: preparation and preliminary SPECT studies in humans
| Autor: | K A, Bergström, J T, Kuikka, A, Ahonen, E, Vanninen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Serotonin Plasma Membrane Transport Proteins Tomography Emission-Computed Single-Photon Dopamine Plasma Membrane Transport Proteins Serotonin Membrane Glycoproteins Dopamine Brain Membrane Transport Proteins Nerve Tissue Proteins Middle Aged Iodine Radioisotopes Cocaine Humans Carrier Proteins |
| Zdroj: | Journal of nuclear biology and medicine (Turin, Italy : 1991). 38(4 Suppl 1) |
| ISSN: | 0368-3249 |
| Popis: | beta-CIT (2 beta-carbomethoxy-3 beta-(4-iodophenyl)tropane) is a new ligand that has a high affinity to dopamine and serotonin re-uptake sites. [123I] beta-CIT was prepared by reacting the corresponding trimethylstannyl precursor with no-carrier-added 123I. Iodogen was used as an oxidizing agent. The labeling mixture was purified by filtration through a mini-column. The purity of the product was confirmed by analytical HPLC. The total radiochemical yield was 67 +/- 5%. The radiochemical purity was95% and the specific activity was107 GBq/mol (2900 Ci/mmol). The final product was confirmed to be free of endotoxins before intravenous administration. Two healthy male volunteers were injected iv with 120-160 MBq of [123I] beta-CIT and scanned with a 3-head gamma-camera (Siemens MultiSPECT3). Dynamic SPECT scans were performed for up to 2 hours. There was a high accumulation of radioactivity in the striatum and in the thalamus, and some in the medial prefrontal area. Thus, we have developed an easy method to prepare [123I] beta-CIT with a high specific radioactivity and in a sufficient radiochemical yield. Specific [123I] beta-CIT binding in striatal and thalamic regions was demonstrated in humans. [123I] beta-CIT is a potential marker of the dopamine and serotonin transporters and can be used to study the pathophysiology of Parkinson's disease, as well as neuropsychiatric disorders. |
| Databáze: | OpenAIRE |
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