| Autor: |
Guangyu, Zhu, Peng, Zhai, Nancy, Wakeham, Xiangyuan, He, Xuejun C, Zhang |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Methods in enzymology. 403 |
| ISSN: |
0076-6879 |
| Popis: |
GGAs are a family of adaptor proteins involved in vesicular transport. As an effector of the small GTPase Arf, GGA interacts using its GAT domain with the GTP-bound form of Arf. The GAT domain is also found to interact with ubiquitin and rabaptin-5. Rabaptin-5 is, in turn, an effector of another small GTPase, Rab5, which regulates early endosome fusion. The interaction between GGAs and rabaptin-5 is likely to take place in a pathway between the trans-Golgi network and early endosomes. This chapter describes in vitro biochemical characterization of the interaction between the GGA1 GAT domain and rabaptin-5. Combining with the complex crystal structure, we reveal that the binding mode is helix bundle-to-helix bundle in nature. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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