| Autor: |
Zhihui, Liu, Zongxiang, Chen, Jingya, Di, Zhisong, Wang, Yongxia, Zhang, Jinshan, Li, Liyou, An, Giorgio Antonio, Presicce, Lanjun, Liu, Fuliang, Du |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Am J Transl Res |
| ISSN: |
1943-8141 |
| Popis: |
We injected mouse zygotes with combinations of Cas9 protein, Cas9 mRNA, and two gRNAs targeting a single exon of type I interferon receptor (IFNAR1) to determine the gene targeting efficiencies. Cas9 protein produced on-target mutations more efficiently than Cas9 mRNA when each was used with a single gRNA, regardless of which gRNA was used. When Cas9 mRNA and Cas9 protein were co-injected, the on-target efficiency could reach 97.0% when both gRNAs were used, which was higher than when either gRNA was used alone (61.3% and 75.5%, respectively; P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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