FlyGEM, a full transcriptome array platform for the Drosophila community

Autor:	Rick, Johnston, Bruce, Wang, Rachel, Nuttall, Michael, Doctolero, Pamela, Edwards, Jining, Lü, Marina, Vainer, Huibin, Yue, Xinhao, Wang, James, Minor, Cathy, Chan, Alex, Lash, Thomas, Goralski, Michael, Parisi, Brian, Oliver, Scott, Eastman
Rok vydání:	2003
Předmět:	DNA Complementary Genome Gene Expression Profiling Nucleic Acid Hybridization Reproducibility of Results Method Genes Insect DNA Exons Blotting Northern Drosophila melanogaster Animals RNA DNA Probes DNA Primers Fluorescent Dyes Oligonucleotide Array Sequence Analysis
Zdroj:	Genome Biology
ISSN:	1474-760X
Popis:	An array, FlyGEM, has been constructed based on amplification of exons from Drosophila genomic DNA. The array gene list has been extensively annotated and linked to other databases. We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8fe0d61cd5b19ed655b8463c269a047f https://pubmed.ncbi.nlm.nih.gov/15003122 Zobrazit plný text záznamu Full text from SpringerLink