[Comparative investigation of antigens associated with plasmatic membranes of rat hepatoma and myogenic cells using anti-kidney serum]
| Autor: | N P, Teriukova, A S, Desheva, G I, Blinova, O A, Mirgorodskaia, V A, Ivanov |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Myoblasts Skeletal Cell Membrane Immunoblotting Molecular Sequence Data Cell Differentiation Kidney Actins Rats Cell Transformation Neoplastic Liver Neoplasms Experimental Antibody Specificity Antigens Neoplasm Organ Specificity Cell Line Tumor Animals Amino Acid Sequence Laminin Fluorescent Antibody Technique Indirect Cell Proliferation |
| Zdroj: | Tsitologiia. 49(11) |
| ISSN: | 0041-3771 |
| Popis: | The investigation of antigenic diversion of tumor cells resulting from the expression of heteroorganic antigens has been continued. Tumor-associated heteroorganic antigens with mol. weight 200-210 kDa (identified before as laminin), 105-130, 75-80 and 43 kDa were detected by anti-kidney serum in fractions of plasmatic membranes of cells of rat ascitic Zajdela hepatoma and cultured HTC hepatoma; the antigen 43 kDa was isolated on immunosorbent and identified by mass spectrometry as beta-actin. Anti-kidney serum revealed laminin in fractions of plasmatic membranes of cultured L8 and L6J1 myoblasts, and L6J1 myotubes; apparently, synthesis of laminin by hepatoma and myogenic cells is not connected with their proliferative activity. Besides, anti-kidney serum detected components 38, 42, 44, 48, 62, 78 and 120 kDa, expression of which on myogenic cells surface might be consequence of active cell proliferation and (or) differentiation. |
| Databáze: | OpenAIRE |
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