| Autor: |
Xing, Li, Wei, Pan, Feng, Qiu, Kun-Lun, Tian, Zong-Yin, Qiu |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. 13(4) |
| ISSN: |
1007-3418 |
| Popis: |
To seek a better profiling of proteins of hepatoma cells.The homogenate of hepatoma cells QGY-7703 was fractionated into four parts by differential centrifugation: the nuclei, the pellet by 20,000 x g, the pellet by 100,000 x g and the cytosolic supernatant. The four fractions were submitted to two-dimensional gel electrophoresis and their electrophoretic patterns were analyzed.In comparison with the protein pattern of hepatoma cells not fractionated, the patterns of the four fractions display many more protein spots, and a large number of proteins present in the nuclei and cytosolic supernatant were not shown in the not-fractionated samples.Preparation of subcellular fractions before electrophoretic procedures proves to be very useful; not only can it improve the results of two-dimensional gel electrophoresis, but also can lead to research into the subcellular level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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