[A novel method of subtraction cDNA hybridization allowing cloning of genes showing differential expression in cell micropopulations]

Autor:	O L, Vasil'ev, S A, Luk'ianov, A V, Beliavskiĭ, O V, Kazanskaia, A G, Zaraĭskiĭ
Rok vydání:	1996
Předmět:	Xenopus laevis DNA Complementary Base Sequence Molecular Sequence Data Genes Homeobox Animals Gene Expression Nucleic Acid Hybridization Amino Acid Sequence Cloning Molecular Polymerase Chain Reaction
Zdroj:	Bioorganicheskaia khimiia. 22(12)
ISSN:	0132-3423
Popis:	A novel, efficient and simple technique that combines subtractive hybridization with kinetic enrichment is proposed for obtaining enriched cDNA. The method is based on the use of a set of special primers that allow for the selective amplification by PCR only of differentially distributed sequences. Using the proposed technique, cDNA of a new gene XEp-1, specifically expressed in the presumptive epidermis of Xenopus laevis, was cloned, starting with the stage of midgastrula.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8f4410ee29e277d1d88554ffebfbb6e3 https://pubmed.ncbi.nlm.nih.gov/9054339 Zobrazit plný text záznamu