Up-regulation of cathepsin X in prostate cancer and prostatic intraepithelial neoplasia
| Autor: | Dorit K, Nägler, Sabine, Krüger, Angela, Kellner, Edmund, Ziomek, Robert, Menard, Peter, Buhtz, Matthias, Krams, Albert, Roessner, Udo, Kellner |
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| Rok vydání: | 2004 |
| Předmět: |
Aged
80 and over Male Prostatectomy Prostatic Intraepithelial Neoplasia Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Blotting Western Cathepsin K Prostatic Neoplasms Middle Aged Cathepsins Immunohistochemistry Up-Regulation Cysteine Endopeptidases Humans RNA Messenger In Situ Hybridization Aged |
| Zdroj: | The Prostate. 60(2) |
| ISSN: | 0270-4137 |
| Popis: | Evidence is accumulating that several proteases are involved in prostate cancer progression. A locus which is often amplified in prostate cancer is the chromosomal region 20q13. Interestingly, one of the genes encoding the cysteine protease cathepsin X maps to this region. The aim of this study was to assess the expression pattern of cathepsin X in malignant and non-malignant prostatic tissue samples.Matched malignant and non-malignant tissue specimens were obtained from 56 men after radical prostatectomy. Cathepsin X was quantified at both protein and mRNA levels using several detection methods: Western blotting, immunohistochemistry, quantitative RT-PCR, and in situ hybridization. Furthermore, genomic DNA was analyzed by PCR for possible gene amplification.Immunohistochemical analysis of formalin-fixed, paraffin-embedded sections of radical prostatectomy specimens was performed utilizing a polyclonal antibody against human procathepsin X and revealed staining of acinar basal cells in normal prostate glands. Prostatic intraepithelial neoplasias (PINs) and prostate carcinomas stained highly positive for cathepsin X, showing a significant difference to the staining of normal prostate glands. In contrast, relatively weak and heterogeneous staining was observed for cathepsins F, B, and L. Up-regulation of cathepsin X at the protein level was confirmed by Western blotting. No statistically significant difference was observed at the mRNA level. PCR of genomic DNA revealed that cathepsin X up-regulation most likely occurs in the absence of genomic amplification.The high expression levels of cathepsin X both in PIN and invasive adenocarcinomas of the prostate suggest that cathepsin X may play a role in the early tumorigenesis of prostate cancer. Further studies are needed to define the utility of this cysteine protease as a diagnostic marker for the early detection of prostate cancer. |
| Databáze: | OpenAIRE |
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