| Autor: |
Daniela, Mayr, Julia, Preishuber-Pflügl, Andreas, Koller, Susanne M, Brunner, Christian, Runge, Anja-Maria, Ladek, Francisco J, Rivera, Herbert A, Reitsamer, Andrea, Trost |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Current eye research. 47(4) |
| ISSN: |
1460-2202 |
| Popis: |
Pericytes (PCs), located abluminal of endothelial cells on capillaries, are essential for vascular development and stability. They display a heterogeneous morphology depending on organ localization, differentiation state, and function. Consequently, PCs show a diverse gene expression profile, impeding the usage of a unique PC marker and therefore the distinct identification of PCs. Inducible reporter mouse models represent an important tool for investigating the fate of PCs under physiological and pathophysiological conditions. PC-specific expression efficiency of the fluorescence reporter tdTomato following tamoxifen induction was analyzed and compared in two inducible Cre recombinase-expressing mouse models under control of the NG2 and PDGFRb promotor.The NG2-CreER™-tdTomato and the PDGFRb-P2A-CreERTamoxifen induction at the three different time points resulted in PC-specific expression of tdTomato in both reporter models. In the NG2-CreER™-tdTomato mouse, the induction efficiency ranged from 21.9 to 35.5%. In the PDGFRb-P2A-CreERBoth reporter mouse models represent promising tools for fate-mapping studies of PCs. While the NG2-CreER™-tdTomato mouse reveals very specific labeling of PCs in the retina, its induction efficiency is lower compared to the PDGFRb-P2A-CreER |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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