| Autor: |
Yu-jia, Liu, Ping, Su, Xiu-juan, Wang, Yu-jun, Zhao, Yu-ru, Tong, Tian-yuan, Hu, Chen, Liu, Wei, Gao, Lu-qi, Huang |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Yao xue xue bao = Acta pharmaceutica Sinica. 51(4) |
| ISSN: |
0513-4870 |
| Popis: |
In this paper, we cloned the full-length cDNA of TwSQS from Tripterygium wilfordii suspension cells(Gen Bank: KR401220) and performed the bioinformation and m RNA expression analysis. The expression after methyl jasmonate(MJ) treatment of the gene was detected by RT-PCR. The full-length cDNA of TwSQS was 1 800 bp containing a 1 242 bp open reading frame(ORF) encoding a polypeptide of 413 amino acids. The theoretical isoelectric point(p I) was 7.94 and the calculate molecular weight was about 47.20 k D. The relative expression level of TwSQS was deduced by MJ and reached the highest at 4 h after the treatment. The gene information we got in this study enriched the biosynthesis pathway of triterpenoids in Tripterygium wilfordii and laid foundation for further studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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