Aldose reductase inhibitor counteracts the enhanced expression of matrix metalloproteinase-10 and improves corneal wound healing in galactose-fed rats
| Autor: | Takamura, Yoshihiro, Matsumoto, Takafumi, Tomomatsu, Takeshi, Matsumura, Takehiro, Takihara, Yuji, Inatani, Masaru |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Corneal Dystrophies
Hereditary Male Wound Healing Integrin alpha3 Administration Topical Epithelium Corneal Administration Oral Galactose Diabetes Mellitus Experimental Diet Rats Rats Sprague-Dawley Gene Expression Regulation Matrix Metalloproteinase 10 Aldehyde Reductase Galactitol Animals Enzyme Inhibitors Research Article Signal Transduction |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose We investigated the effect of an aldose reductase inhibitor (ARI) and the role of matrix metalloproteinase (MMP)-10 on recovery after corneal epithelium removal in a rat diabetic keratopathy model. Methods Three-week-old Sprague-Dawley rats were fed the following diets for 6 weeks: normal MF chow (MF), 50% galactose (Gal), and 50% Gal containing 0.01% ARI (Gal +ARI). The corneal epithelium was removed using n-heptanol, and the area of epithelial defects was photographed and measured every 24 h. Real-time reverse transcriptase PCR, western blotting, and immunohistochemistry were used to determine the expression profile of MMP-10 and integrin α3. Results Compared to the MF control group, the amount of galactitol in the Gal group increased approximately 200-fold, which was reduced to sevenfold by ARI treatment. The area of corneal erosion in the Gal group was significantly larger than in the MF group at 72 h and thereafter (p |
| Databáze: | OpenAIRE |
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