| Autor: |
M, Gerbal, P, Fournier, P, Barry, M, Mariller, F, Odier, G, Devauchelle, M, Duonor-Cerutti |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
In vitro cellulardevelopmental biology. Animal. 36(2) |
| ISSN: |
1071-2690 |
| Popis: |
Sf21 and Sf9 cell lines established from the lepidoptera Spodoptera frugiperda do not display major induction of heat shock proteins when exposed to a temperature of 37 degrees C. After some months of adaptation at 37 degrees C we obtained two new cell lines, Sf21-HT and Sf9-HT, which have now been established for several years in our laboratory. The Sf9-HT line displays a slightly shorter doubling time at 37 degrees C than the wild type at 28 degrees C, but cell lethality gives rise to an earlier growth arrest. The composition of total lipid extract from heat-adapted cells reveals a higher sphingomyelin to phosphatidylcholine ratio and a higher percentage of saturated fatty acids, which are expected for the lower membrane fluidity, required for thermotolerance. The cell volume of Sf9-HT is doubled, and by flow cytometry we showed that the DNA content is twice that in the parental cell line. Karyotypic examination of metaphasic cells achieved under epifluorescence microscopy revealed a doubled chromosome number in Sf9-HT. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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