Effects of sargentgloryvine stem extracts on HepG-2 cells in vitro and in vivo
| Autor: | Ming-Hua, Wang, Min, Long, Bao-Yi, Zhu, Shu-Hui, Yang, Ji-Hong, Ren, Hui-Zhong, Zhang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Mice Inbred BALB C Plant Stems Brief Article Plant Extracts bcl-X Protein Cytochromes c Gene Expression Mice Nude Apoptosis Hep G2 Cells Xenograft Model Antitumor Assays Mice bcl-2 Homologous Antagonist-Killer Protein Proto-Oncogene Proteins c-bcl-2 Caspases Animals Humans Myeloid Cell Leukemia Sequence 1 Protein |
| Zdroj: | World journal of gastroenterology. 17(23) |
| ISSN: | 2219-2840 |
| Popis: | To observe the effects of sargentgloryvine stem extracts (SSE) on the hepatoma cell line HepG-2 in vitro and in vivo and determine its mechanisms of action.Cultured HepG-2 cells treated with SSE were analysed by 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-Diphenyltetrazolium bromide and clone formation assay. The cell cycle and apoptosis analysis were conducted by flow cytometric, TdT-Mediated dUTP Nick End Labeling and acridine orange/ethidium bromide staining methods, and protein expression was examined by both reverse transcriptase-polymerase chain reaction and Western blotting. The pathological changes of the tumor cells were observed by haematoxylin and eosin staining. Tumor growth inhibition and side effects were determined in a xenograft mouse model.SSE treatment could not only inhibit HepG-2 cell proliferation in a dose- and time-dependent manner but also induce apoptosis and cell cycle arrest at the S phase. The number of colonies formed by SSE-treated tumor cells was fewer than that of the controls (P0.05). SSE induced caspase-dependent apoptosis accompanied by a significant decrease in Bcl-xl and Mcl-1 and elevation of Bak expression (P0.05). Tumor necrosis factor α in the xenograft tumor tissue and the liver functions of SSE-treated mice showed no significant changes at week 8 compared with the control group (P0.05). Systemic administration of SSE could inhibit the HepG-2 xenograft tumor growth with no obvious toxic side effects on normal tissues.SSE can induce apoptosis of HepG-2 cells in vitro and in vivo through decreasing expression of Bcl-xl and Mcl-1 and increasing expression of Bax. |
| Databáze: | OpenAIRE |
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