X-ray film digestion: a semi-quantitative assay for proteolytic enzymes used in immunohaematology

Autor:	J M, Giles, D J, Rogers
Rok vydání:	1990
Předmět:	Cysteine Endopeptidases Blood Grouping and Crossmatching X-Ray Film Humans Clinical Enzyme Tests
Zdroj:	Medical laboratory sciences. 47(3)
ISSN:	0308-3616
Popis:	A simple semi-quantitative assay method for proteolytic enzyme solutions used in blood group serology is described. The method is an adaptation of a technique formerly used for assay of faecal trypsin and is based on the digestion of the gelatin coating of unexposed X-ray film. A Spearman rank correlation coefficient of 0.8 was obtained when the X-ray film technique was compared with a spectrophotometric method using azoalbumin as substrate. The method is not suitable as a reference technique or as a substitute for serological control, but is useful for monitoring or comparing enzyme preparations in some circumstances or situations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8dcdf7e60f025c4853db59d898d8e447 https://pubmed.ncbi.nlm.nih.gov/2402198 Zobrazit plný text záznamu