| Autor: |
G G, Charkviani, A V, Kulikov, T M, Eristavi, Z O, Dzhaparidze |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Biofizika. 26(5) |
| ISSN: |
0006-3029 |
| Popis: |
Interlocation of sulfhydryl groups S1 and corresponding divalent ions binding sites in myosin has been studied. S1-SH groups were labelled with iodacetamide spin labels and their saturation curves were measured in the presence of Ca2+ and Mn2+. The spin-lattice relaxation rates 1/T1 of labels were calculated from saturation curves and according to their changes caused by replacing Ca2+ by Mn2+ the distance between sulfhydryl groups S1 and divalent ions binding sites were estimated to be 45 +/- 2.5 A. The addition of ATP to this system has not changed the character of interaction. An analysis of saturation curves measured within temperature range 20-100 K suggests that two myosin subfragments J differ in conformation at least in the range where S1-SH groups are located. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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