| Autor: |
F, Larrea, A, Martínez-Castillo, V, Cabrera, J, Alcocer-Varela, G, Queipo, C, Cariño, D, Alarcón-Segovia |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
The Journal of clinical endocrinology and metabolism. 82(11) |
| ISSN: |
0021-972X |
| Popis: |
We have evaluated the production of PRL by human peripheral mononuclear cells (PBMNC) from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P0.001) amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned medium was inhibited with specific antiserum to human PRL. Western blotting using a polyclonal antibody to human PRL revealed a single 60-kDa PRL-like species in both normal and SLE PBMNC extracts, the immunoreactivity of which was preferentially found in SLE subjects. With the use of reverse transcription-PCR an expected 633-bp band was observed, and its similarity to pituitary PRL was further confirmed by Southern blot analysis with human PRL complementary DNA as a probe. We conclude that a high molecular mass PRL-like species is synthesized and secreted by PBMNC, and patients with SLE have an increased secretion of lymphocyte-derived PRL-like material. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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