| Autor: |
A A, Markarian, T D, Dargaeva, R N, Aliautdin, V L, Burkov |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Biomeditsinskaia khimiia. 50(2) |
| ISSN: |
2310-6972 |
| Popis: |
A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sequential cascade of 1.0-5.0 kDa ultrafiltration and the following subsequent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0.5-0.6% of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0.85 +/- 0.06 A280/g dry extract) within 8-9 hours. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
