| Autor: |
M G, Isaguliants, Iu P, Kadoshnikov, T I, Kalinina, Iu E, Khuliakov, Iu A, Semiletov, V D, Smirnov, B, Wahren |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 61(3) |
| ISSN: |
0320-9725 |
| Popis: |
Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesized using two expression systems-one based on the thermoinducible PR promoter of bacteriophage lambda and the other one based on phi 10 promoter of bacteriophage T7 with 3-5% and 7-14% yields, respectively. The hybrids have dual HBV and HIV-1 immunospecificity and are assembled into particles similar to those formed by the protein carrier HBcAg. Sandwich ELISA and immune electron microscopy revealed that HIV-1 epitopes are exposed on the surface of the particles. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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