Tracking of single tRNAs for translation kinetics measurements in chloramphenicol treated bacteria
| Autor: | Ivan L, Volkov, A Carolin, Seefeldt, Magnus, Johansson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Intravital Microscopy Escherichia coli Proteins Chloramphenicol Resistance Recombinant Proteins Single Molecule Imaging Anti-Bacterial Agents Kinetics RNA Bacterial Chloramphenicol Electroporation Microscopy Fluorescence RNA Transfer Protein Biosynthesis Escherichia coli Ribosomes |
| Zdroj: | Methods (San Diego, Calif.). |
| ISSN: | 1095-9130 |
| Popis: | Chloramphenicol is a broad-spectrum antibiotic targeting the protein synthesis machinery by binding to the bacterial ribosome. Chloramphenicol has been considered a classic general inhibitor of translation, blocking the accommodation of aa-tRNA into the A site of the large ribosomal subunit. However, recent studies suggest that this proposed mechanism is a simplification and that the effect of chloramphenicol on mRNA translation is much more dynamic. By tracking single dye-labelled elongator and initiator tRNAs in Escherichia coli cells treated with chloramphenicol, we observe the direct effect of chloramphenicol on translation kinetics. We find clear indications of slow but significant mRNA translation on drug bound ribosomes. |
| Databáze: | OpenAIRE |
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