TNF-alpha enhances colony-stimulating factor-1-induced macrophage accumulation in autoimmune renal disease
| Autor: | K J, Moore, K, Yeh, T, Naito, V R, Kelley |
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| Rok vydání: | 1996 |
| Předmět: |
Mice
Inbred BALB C Mice Inbred C3H Tumor Necrosis Factor-alpha Macrophage Colony-Stimulating Factor Macrophages Kidney Glomerulus Gene Transfer Techniques Lupus Nephritis Epithelium Mice Mutant Strains Colony-Forming Units Assay Mice Kidney Tubules Adjuvants Immunologic Bone Marrow Animals Female Cell Division |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 157(1) |
| ISSN: | 0022-1767 |
| Popis: | The lpr mutation on the MRL background accelerates autoimmune nephritis in which macrophage (M phi) accumulation is prominent. Renal disease is absent in other strains with lpr. TNF-alpha and CSF-1 are increased in the kidney of MRL-lpr mice with loss of renal function. We have established that CSF-1 can incite renal injury in mice with the lpr mutation, and M phi from the MRL strain hyper-respond to this growth factor. We hypothesized that TNF-alpha enhanced the M phi response to CSF-1 in MRL-lpr mice. We now report that TNF-alpha enhanced CSF-1-induced bone marrow M phi proliferation in MRL-lpr mice, and not in congenic MRL +/+, normal C3H +/+, and BALB/c, or another strain with lpr (C3H-lpr). Using a gene transfer approach to deliver CSF-1 together with TNF-alpha into the kidney, we evaluated the impact on renal injury. Tubular epithelial cells genetically modified to produce CSF-1 (CSF-1-TEC) and TNF-alpha (TNF-TEC) placed under the renal capsule caused a greater accumulation of M phi in the implant site than CSF-1-TECs alone in MRL-lpr, but not MRL +/+ mice. We noted in tissues adjacent but not distal to the implanted TECs, an increase in M phi in the interstitium and surrounding glomeruli of MRL-lpr but not MRL +/+ mice. This indicated that CSF-1 and TNF-alpha released by TECs were responsible for promoting renal pathology. Taken together, these data suggest that the simultaneous expression of TNF-alpha and CSF-1 in the MRL-lpr kidney fosters M phi accumulation. We speculate that the increase in M phi in the kidney in response to CSF-1 and TNF-alpha is responsible for the rapid tempo of autoimmune renal injury in MRL-lpr mice. |
| Databáze: | OpenAIRE |
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