Rat messenger RNA for the retinal pigment epithelium-specific protein RPE65 gradually accumulates in two weeks from late embryonic days

Autor:	G, Manès, R, Leducq, J, Kucharczak, A, Pagès, C F, Schmitt-Bernard, C P, Hamel
Rok vydání:	1998
Předmět:	cis-trans-Isomerases DNA Complementary Time Factors Molecular Sequence Data Proteins Eye Polymerase Chain Reaction Rats Mice Animals RNA Messenger Rats Wistar Carrier Proteins Eye Proteins Pigment Epithelium of Eye
Zdroj:	FEBS letters. 423(2)
ISSN:	0014-5793
Popis:	The RPE65 protein appears late during the retinal development. To study the basis for this regulation, the rat RPE65 cDNA was sequenced and the mRNA subsequently quantitated at various stages by competitive RT-PCR. RPE65 mRNA was detected as early as E18 (36 copies/ng of whole eye total RNA). It gradually accumulates up to P12 (27000 copies/ng) at which point it reaches a steady state level. This increase is interrupted for 3 days (P2-P4) during which the levels of mRNA remain stable. This timing and rate of accumulation parallels that of rat and mouse opsin mRNA and suggests that common factors may control the activation of genes in photoreceptors and retinal pigment epithelium cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8d3f5ba6034c2676a4f6316bdca034b4 https://pubmed.ncbi.nlm.nih.gov/9512345 Zobrazit plný text záznamu Plný text