| Autor: |
S A, Dambinova, A I, Gorodinskiĭ |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 49(1) |
| ISSN: |
0320-9725 |
| Popis: |
The binding of L-[3H]glutamate to rat cerebral cortex synaptic membranes was investigated. Two types of binding sites, a Na+-independent (Kd = 140-160 nm; Bmax = 3.8-4.5 pmol-mg of protein) and a Na+-dependent (Kd = 2.0 microM; Bmax = 45-50 pmol/mg of protein) ones, were detected. The dependence of Na+-insensitive binding on time and temperature and membrane content in a sample was determined. Mono- and divalent cations (5-10 mM) potentiated specific binding by 2.1-3.3 times. The Na+-dependent binding is associated with active transport systems, while the Na+-independent one-with true receptor binding. The relationship between CNS glutamate receptors and Na+-independent binding sites is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
