| Autor: |
Jesse, Aaron, Elder, de la Rosa, Kort, Travis, Nathan, Harrison, Justin, Burt, Miguel, José-Yacamán, Konstantin, Sokolov |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Optics express. 16(3) |
| ISSN: |
1094-4087 |
| Popis: |
Advances in plasmonic nanoparticle synthesis afford new opportunities for biosensing applications. Here, we apply a combination of a new type of plasmonic nanomaterial - stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio. Depolarization of linear polarized light by stellated nanoparticles is over 15-fold more efficient than similarly-sized spheroidal nanoparticles. This efficient light depolarization allows robust detection of molecules labeled with stellated nanoparticles in cross-polarized imaging where the intrinsic light scattering from cells is significantly reduced. The imaging can be carried out with single molecule sensitivity for essentially unlimited time with no signal degradation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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