| Autor: |
Sohaib Abdul, Rehman, Alexander R, Carr, Martin O, Lenz, Steven F, Lee, Kevin, O'Holleran |
| Rok vydání: |
2018 |
| Zdroj: |
Optics express. 26(4) |
| ISSN: |
1094-4087 |
| Popis: |
Super-resolution techniques that localize single molecules in three dimensions through point spread function (PSF) engineering are very sensitive to aberrations and optical alignment. Here we show how double-helix point spread function is affected by such mis-alignment and aberration. Specifically, we demonstrate through simulation and experiment how misplacement of phase masks in infinity corrected systems is a common source of significant loss of accuracy. We also describe an optimal alignment and calibration procedure to correct for these errors. In combination, these optimizations allow for a maximal field of view with high accuracy and precision. Though discussed with reference to double-helix point spread function (DHPSF), the optimization techniques are equally applicable to other engineered PSFs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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