Rapid and sensitive on-line liquid chromatographic/tandem mass spectrometric determination of an ethylene oxide-DNA adduct, N7-(2-hydroxyethyl)guanine, in urine of nonsmokers

Autor:	Chih-Chun Jean, Huang, Wei-Chung, Shih, Chia-Fang, Wu, Ming-Feng, Chen, Yi-Lung, Chen, Ying-Huei, Lin, Kuen-Yuh, Wu
Rok vydání:	2008
Předmět:	Ethylene Oxide Air Pollutants DNA Adducts Spectrometry Mass Electrospray Ionization Guanine Reference Values Tandem Mass Spectrometry Smoking Humans DNA Biomarkers Chromatography High Pressure Liquid
Zdroj:	Rapid communications in mass spectrometry : RCM. 22(5)
ISSN:	0951-4198
Popis:	Ethylene oxide (EtO) is classified as a known human carcinogen. The formation of EtO-DNA adducts is considered as an important early event in the EtO carcinogenic process. An isotope-dilution on-line solid-phase extraction and liquid chromatography coupled with tandem mass spectrometry method was then developed to analyze one of the EtO-DNA adducts, N7-(2-hydroxyethyl)guanine (N7-HEG), in urine of 46 nonsmokers with excellent accuracy, sensitivity and specificity. The merits of this method include small sample volume (only 120 microL urine required), automated sample cleanup, and short total run time (12 minutes per sample). This method demonstrates its high-throughput capacity for future molecular epidemiology studies on the potential health effects resulting from the low-dose EtO exposure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8c991521b7a62b75a2e17797e3ad6388 https://pubmed.ncbi.nlm.nih.gov/18257114 Zobrazit plný text záznamu Plný text