Monitoring antigen-specific T cells using MHC-Ig dimers
| Autor: | J P, Schneck, J E, Slansky, S M, O'Herrin, T F, Greten |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Complementary Recombinant Fusion Proteins Genetic Vectors Histocompatibility Antigens Class I Histocompatibility Antigens Class II Receptors Antigen T-Cell Immunoglobulins Enzyme-Linked Immunosorbent Assay CD8-Positive T-Lymphocytes Flow Cytometry Polymerase Chain Reaction Antigens Surface Animals Humans Antigens Cloning Molecular Dimerization |
| Zdroj: | Current protocols in immunology. |
| ISSN: | 1934-368X |
| Popis: | The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells. |
| Databáze: | OpenAIRE |
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