| Autor: |
F, Fauvel-Lafève, B, Arbeille, C, de Romeuf, M, Lemesle, Y J, Legrand |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 319(4) |
| ISSN: |
0764-4469 |
| Popis: |
We report the results of an immunogold electron microscopical analysis on microfibrils from the arterial subendothelium showing that thrombospondin (TSP) is present on 40 nm-diameter structures joining 8-10 nm-diameter microfibrils containing fibrillin. They differ from type VI collagen which forms 3-5 nm-diameter microfibrils. TSP containing microfibrils (TSP-MF) extracted from human umbilical arteries did not contain fibrillin or type VI collagen. Blood platelet interactions with TSP-MF were not modified by anti-fibrillin or anti-type VI collagen antibodies. In situ, vWF was bound to cross-linked microfibrils, at the level of their 40 nm junction, and a double-labeling with the anti-thrombospondin and anti-vWF antibodies was observed. In vitro, vWF binding to TSP-MF was not inhibited by anti-fibrillin or anti-type VI collagen antibodies. These results suggest a structural and functional heterogeneity of microfibrils and emphasize the role of TSP-MF in the thrombogenicity of the subendothelium. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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