| Autor: |
Shaikh Shohidul, Islam, Md Rezaul, Karim, A K M, Asaduzzaman, A H M Khurshid, Alam, Zahid Hayat, Mahmud, Syed Rashel, Kabir |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Journal of food biochemistryREFERENCES. 45(5) |
| ISSN: |
1745-4514 |
| Popis: |
Trichosanthes dioica seed lectin (TDSL), having a molecular mass of 57 ± 2 kDa was purified in an alternative way. For the purification process, the galactose-sepharose-4B affinity column was used. The purified TDSL agglutinated human and mouse erythrocytes at the minimum concentration of 8 μg/ml. d-lactose and d-galactose were the most potent inhibitory sugars as observed. The purified lectin was a glycoprotein having 3.0% of a neutral sugar. The lectin exhibited maximum activity up to 60°C and pH range from 7.0 to 10.0 and stable up to 4.0 M urea as tested. The lectin demonstrated mild toxicity when administered against brine shrimp nauplii, and the LC |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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