| Autor: |
Holly C, Gibbs, Sreeja, Sarasamma, Oscar R, Benavides, David G, Green, Nathan A, Hart, Alvin T, Yeh, Kristen C, Maitland, Arne C, Lekven |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2440 |
| ISSN: |
1940-6029 |
| Popis: |
Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB) with light sheet fluorescence microscopy (LSFM) and pilot experiments to create a study-specific set of parameters for semiautomatically tracking cellular movements in the embryonic midbrain primordium during zebrafish segmentation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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