Diagnostic and biological significance of anti-p41 IgM antibodies against Borrelia burgdorferi
| Autor: | E, Ulvestad, A, Kanestrøm, L J, Sønsteby, R, Jureen, T, Omland, B, Edvardsen, J, Lundervik, E, Kristoffersen, A P, van Dam |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Aged 80 and over Male Antigens Bacterial Lyme Disease Adolescent Blotting Western In Vitro Techniques Middle Aged Antibodies Bacterial Immunity Innate Borrelia burgdorferi Group Immunoglobulin M Immunoglobulin G Humans False Positive Reactions Female Child Fluorescent Antibody Technique Indirect Aged Flagellin |
| Zdroj: | Scandinavian journal of immunology. 53(4) |
| ISSN: | 0300-9475 |
| Popis: | We performed a prospective study to investigate the biological significance and diagnostic specificity of anti-p41 immunoglobulin (Ig)M antibodies against Borrelia burgdorferi. During a 1-year interval 2403 patients were referred to our department for B. burgdorferi serology. Sixty-three patients had repetitive positive tests for IgM anti-p41 antibodies and negative tests for anti-p41 IgG antibodies. Ten of the 63 patients recently had symptoms of erythema migrans. A confirmatory IgM Western blot gave a positive reaction in 5 patients out of 53 patients with little or no clinical evidence of B. burgdorferi infection. The remaining 48 patients were negative in this test and were considered as false-positives. Two whole cell enzyme-linked immunosorbent assay (ELISAs), two immunofluorescence assays and Western blotting were not useful as confirmatory tests. Sera from 330 blood donors and 72 cord sera were also screened for anti-p41 IgM. Five blood donor sera and five cord sera showed an IgM reactivity against p41. Based on our data we hypothesize that up to 1.5% of the population may have natural IgM antibodies against p41 in their sera. We observed that six out of nine sera with such antibodies could immobilize a B. afzelii reference strain in vitro. Whether anti-p41 IgM antibodies are capable of inactivating infective spirochetes and thereby prevent infection in vivo is, however, not yet clarified. The paradoxical conclusion that anti-p41 IgM antibodies may be a sign of resistance to infection rather than a sign of infection should be given consideration. |
| Databáze: | OpenAIRE |
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