| Autor: |
A, Milev, K, Karakostov, C, Nikolov, L, Dimitrov, L, Tzonev, T, Tzvetkov, N, Aleksiev |
| Rok vydání: |
1982 |
| Předmět: |
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| Zdroj: |
Folia haematologica (Leipzig, Germany : 1928). 109(3) |
| ISSN: |
0323-4347 |
| Popis: |
Platelet concentrates amounting to 142 X 10(9) +/- 11 X 10(9) cells are prepared by cytopheresis from one liter of blood. Ten of the concentrates are labelled with 51Cr and reinfused autologously. The other ten concentrates are frozen with a controlled rate freezer in a medium containing 10% DMSO and 5% glucose for 7 days at -196 degrees C. The fresh platelets have a 51Cr 24 hrs. recovery of 68 +/- 7% and a survival rate (T 1/2) of 10 +/- 1 days. The frozen preserved platelets have a 51Cr 24 hrs. recovery of 54 +/- 6.1%, a T 1/2 of 8.1 days, and significantly increased liver sequestration. There is a correlation between the reversal reaction in vitro after hypotonic shock and the 51Cr 24 hrs. in vivo recovery of fresh platelets and the 51Cr 1st hr. recovery of frozen platelets. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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