On-chip chiral and achiral separation of amphetamine and related compounds labeled with 4-fluoro-7-nitrobenzofurazane

Autor:	S R, Wallenborg, I S, Lurie, D W, Arnold, C G, Bailey
Rok vydání:	2000
Předmět:	Ephedrine Amphetamine Propiophenones Structure-Activity Relationship 4-Chloro-7-nitrobenzofurazan Amphetamines Phenylpropanolamine Electrophoresis Capillary Stereoisomerism Fluorescent Dyes Methamphetamine
Zdroj:	Electrophoresis. 21(15)
ISSN:	0173-0835
Popis:	Amphetamine and analogous compounds have been labeled with 4-fluoro-7-nitrobenzofurazane and analyzed on a microfabricated chip. Separation of norephedrine, ephedrine, cathinone, pseudoephedrine, methcathinone, amphetamine and methamphetamine is demonstrated using micellar electrokinetic capillary chromatography (MEKC) and laser-induced fluorescence (LIF) detection. Chiral separations of individual drugs were studied using neutral and negatively charged cyclodextrins (CDs) with and without the addition of an organic modifier and/or sodium dodecyl sulfate (SDS). The best results were obtained using a highly sulfated gamma-CD (HS-gamm-CD) in combination with a low concentration of SDS. To obtain complete separation of a mixture of (+/-)-norephedrine, (+/-)ephedrine, (+/-)-pseudoephedrine, (+/-)-methcathinone, (+/-)-amphetamine and (+/-)-methamphetamine it was necessary to add a small amount (1.5 mM) of SDS to the separation buffer. Optimized chiral separation was achieved within 7 min using an S-folded separation channel, a separation voltage of 8 kV and a buffer consisting of 50 mM phosphate (pH 7.35), 10 mM HS-gamma-CD and 1.5 mM SDS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8b7b64bc289123e27e3235bb678f2c66 https://pubmed.ncbi.nlm.nih.gov/11001224 Zobrazit plný text záznamu Plný text