The complete amino acid sequence of subunit d of rat liver mitochondrial H(+)-ATP synthase
| Autor: | T, Higuti, K, Kuroiwa, S, Miyazaki, Y, Yoshihara, H, Toda, T, Kakuno, F, Sakiyama |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Complementary Base Sequence Sequence Homology Amino Acid Molecular Sequence Data DNA Recombinant Mitochondria Liver Rats Molecular Weight Proton-Translocating ATPases Liver Neoplasms Experimental Tumor Cells Cultured Animals RNA Amino Acid Sequence RNA Messenger Oligonucleotide Probes Poly A Chromatography High Pressure Liquid |
| Zdroj: | Journal of biochemistry. 114(5) |
| ISSN: | 0021-924X |
| Popis: | Subunit d of H(+)-ATP synthase from rat liver mitochondria was isolated from the purified enzyme by reverse-phase high performance liquid chromatography. The partial amino acid sequence of the subunit was determined by automated Edman degradation of the peptide fragments. The nucleotide sequence of subunit d of rat liver H(+)-ATP synthase was determined from a recombinant cDNA clone isolated by screening a rat hepatoma cell line H4TG cDNA library with a probe DNA. The sequence was composed of 581 nucleotides including a coding region for the import precursor of subunit d and noncoding regions on the 5'- and 3'- sides. The possible precursor of subunit d and its mature polypeptide deduced from the open reading frame consisted of 161 and 160 amino acid residues with molecular weights of 18,763 and 18,631, respectively. Subunit d is a hydrophilic protein with an isoelectric point of 6.19. The sequence of the rat subunit d is highly homologous with that of subunit d of bovine heart and slightly similar to that of the subunit d of the yeast mitochondria. However, it had no homology with the sequence of any of the subunits of bacterial or chloroplast H(+)-ATP synthase. |
| Databáze: | OpenAIRE |
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