High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection

Autor:	Jana, Olsovská, Markéta, Jelínková, Petr, Man, Markéta, Koberská, Jirí, Janata, Miroslav, Flieger
Rok vydání:	2006
Předmět:	Organisms Genetically Modified Fermentation Spectrophotometry Ultraviolet Sensitivity and Specificity Chromatography High Pressure Liquid Streptomyces Chromatography Liquid Lincomycin
Zdroj:	Journal of chromatography. A. 1139(2)
ISSN:	0021-9673
Popis:	A new separation and quantification method using ultra-performance liquid chromatography (UPLC) with UV detection was developed for detection of lincomycin traces in fermentation broth of different Streptomyces spp. A similar high-performance liquid chromatography (HPLC) protocol was simultaneously developed for comparison purposes. Both methods were validated and showed a linear range of detector response for quantification of lincomycin in concentration from 3.125 to 1000.0 microgml(-1) with correlation coefficient 0.999 and recoveries ranging from 81.5 to 89.85% with precisionor =5%. Compared with the HPLC, the UPLC method offered high sample throughput and about 10 times lower consumption of solvents. The developed assays were used for determination of lincomycin production in genetically manipulated production strain Streptomyces lincolnensis and for determination of lincomycin production after heterologous expression of lincomycin biosynthetic gene cluster in non-producing strain Streptomyces coelicolor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8b6ce595660748992b1d9ac3ea1afaf5 https://pubmed.ncbi.nlm.nih.gov/17125778 Zobrazit plný text záznamu Full Text from ScienceDirect