Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR
| Autor: | J, Bojunga, S, Röddiger, M, Stanisch, K, Kusterer, R, Kurek, H, Renneberg, S, Adams, E, Lindhorst, K H, Usadel, P M, Schumm-Draeger |
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| Rok vydání: | 2000 |
| Předmět: |
endocrine system
endocrine system diseases Goiter Reverse Transcriptase Polymerase Chain Reaction polymerase chain reaction Thyroiditis Autoimmune Regular Article reverse transcription Sensitivity and Specificity Thyroglobulin Thyroid Diseases Graves Disease molecular diagnostics thyroglobulin mRNA Organ Specificity Case-Control Studies thyroid cancer Humans RNA Messenger Thyroid Neoplasms |
| Zdroj: | British Journal of Cancer |
| ISSN: | 0007-0920 |
| Popis: | The sensitive detection of circulating tumour cells in patients with differentiated thyroid cancer may precede the detection of relapse by other diagnostic studies – such as serum thyroglobulin – and thus may have important therapeutic and prognostic implications. We performed reverse transcription-polymerase chain reaction (RT-PCR) on blood samples from patients diagnosed with thyroid disease using two different RT-PCR sensitivities. Additionally, tissue specificity of TG mRNA-expression was determined using RNA extracts from 27 different human tissues. The lower limit of detection was 50–100 TG mRNA producing cells/ml blood using a ‘normal’ RT-PCR sensitivity and 10–20 cells/ml blood using a ‘high’ sensitivity. With the normal sensitivity TG mRNA was detected in 9/13 patients with thyroid cancer and metastasis, 63/137 patients with a history of thyroid cancer and no metastasis, 21/85 with non-malignant thyroid disease and 9/50 controls. With the high sensitivity TG mRNA was detected in 11/13 patients with thyroid cancer and metastasis, 111/137 patients with a history of thyroid cancer and no metastasis, 61/85 with non-malignant thyroid disease and 41/50 controls. Interestingly, using the normal RT-PCR sensitivity TG mRNA transcripts are specific for thyroid tissue and detectable in the peripheral blood of controls and patients with thyroid disease, which correlates with a diagnosis of metastasized thyroid cancer. However, with a high RT-PCR sensitivity, TG mRNA expression was found not to be specific for thyroid tissue and was not correlated with a diagnosis of thyroid cancer in patients. As a consequence, to date TG mRNA detected by RT-PCR in the peripheral blood cannot be recommended as a tumour marker superior to TG serum-level. © 2000 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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