Blockade of αEβ7 integrin suppresses accumulation of CD8
| Autor: | Sebastian, Zundler, Daniela, Schillinger, Anika, Fischer, Raja, Atreya, Rocío, López-Posadas, Alastair, Watson, Clemens, Neufert, Imke, Atreya, Markus F, Neurath |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Mice Knockout Integrins T-Lymphocytes T-Lymphocytes Helper-Inducer CD8-Positive T-Lymphocytes Middle Aged Antibodies Monoclonal Humanized Flow Cytometry Inflammatory Bowel Diseases Immunohistochemistry Mice Gastrointestinal Agents Animals Humans Female Biomarkers |
| Zdroj: | Gut. 66(11) |
| ISSN: | 1468-3288 |
| Popis: | Therapeutically targeting lymphocyte adhesion is of increasing relevance in IBD. Yet, central aspects of the action of antiadhesion compounds are incompletely understood. We investigated the role of αEβ7 and α4β7 integrins and their blockade by vedolizumab and etrolizumab for trafficking of IBD T lymphocytes in an in vivo model of homing to and retention in the inflamed gut.We explored integrin expression in patients with IBD by flow cytometry and immunohistochemistry, while regulation of integrins was studied in T cell cultures. The functional relevance of integrins was assessed by adhesion assays and a recently established humanised mouse model in dextran sodium sulfate-treated immunodeficient mice.High expression of αEβ7 was noted on CD8AEβ7 is of key relevance for gut trafficking of IBD CD8 |
| Databáze: | OpenAIRE |
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