Exopolysaccharide from
| Autor: | Mallory L, Paynich, Sara E, Jones-Burrage, Katherine L, Knight |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Macrophages
T-Lymphocytes Polysaccharides Bacterial Enterobacteriaceae Infections Enzyme-Linked Immunosorbent Assay Colitis Flow Cytometry Lymphocyte Activation Real-Time Polymerase Chain Reaction Adoptive Transfer Article Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal Mice Animals Citrobacter rodentium Bacillus subtilis |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 198(7) |
| ISSN: | 1550-6606 |
| Popis: | Commensal bacteria contribute to immune homeostasis in the gastrointestinal tract; however, the underlying mechanisms for this are not well understood. A single dose of exopolysaccharide (EPS) from the probiotic spore-forming bacterium Bacillus subtilis protects mice from acute colitis induced by the enteric pathogen Citrobacter rodentium. Adoptive transfer of macrophage-rich peritoneal cells from EPS-treated mice confers protection from disease to recipient mice. In vivo, EPS induces development of anti-inflammatory M2 macrophages in a TLR4-dependent manner, and these cells inhibit T cell activation both in vitro and in C. rodentium-infected mice. In vitro, the M2 macrophages inhibit both CD4+ and CD8+ T cells, and the inhibition of CD4+ T cells is dependent on TGF-β, whereas inhibition of CD8+ T cells is dependent on both TGF-β and PD-L1. We suggest that administration of B. subtilis EPS can be utilized to broadly inhibit T cell activation and thus control T cell-mediated immune responses in numerous inflammatory diseases. |
| Databáze: | OpenAIRE |
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