| Autor: |
I N, D'iakov, V S, Pokrovskiĭ, E P, Sannikova, N V, Bulushova, M V, Pokrovskaia, S S, Aleksandrova |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (6) |
| ISSN: |
0372-9311 |
| Popis: |
Evaluate immune response in mice against various L-asparaginases and determine their cross-immunogenicity.The studies were carried out in C57Bl(6j) line mice. Immunogenicity of L-asparaginases was studied: Escherichia coli type II (recombinant) (Medak, Germany) (EcA); Erwinia carotovora type II (ErA); Yersinia pseudotuberculosis type II (YpA); Rhodospirillum rubrum type I (RrA); Wollinella succinogenes type II (WsA). Immune response against the administered antigens was determined in EIA.Y. pseudotuberculosis L-asparaginase was the most immunogenic, E. coli--the least immunogenic. E. carotovora, R. rubrum, W. succinogenes asparaginases displayed intermediate immunogenicity. The results of cross-immunogenicity evaluation have established, that blood sera of mice, that had received YpA, showed cross-immunogenicity against all the other L-asparaginase preparations except E. carotovora. During immunization with E. coli L-asparaginase the developed antibodies also bound preparation from E. carotovora. Sera from mice immunized with W. succinogenes, E. carotovora and R. rubrum L-asparaginases had cross-reaction only with EcA and did not react with other preparations.Cross-immunogenicity of the studied L-asparaginases was determined. A sequence of administration of the studied preparation is proposed that allows to minimize L-asparaginase neutralization by cross-reacting antibodies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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